Fern Culture Media

Fern Culture Media kdorfman Thu, 01/11/2024 - 19:16

C Fern Manual

Carolina C-Fern materials

Pre-made fern medium, Carolina 156780, sold in 60 mL or 400 mL

Carolina recommends 10 mL per plate. I use 9 mL

To get gametophytes in all stages, sow fern spores in 12 60 mm dishes 4 times:

  • 1/15
  • 1/22
  • 1/29
  • 2/2 or Friday 2/3
C-Fern Manual

Fern Medium from scratch

Fern Medium from scratch kdorfman Fri, 01/12/2024 - 21:18

To make one liter
800 mL water
10X macronutrients: 100mL
200X micronutrients: 5 mL
100X Chelated Iron solution: 10 mL

pH to 6 with NaOH

Bring final volume to 1 L

Add 10 g Bacto agar (others may not work)

Autoclave 15 min

10X fern macronutrients

10X fern macronutrients kdorfman Fri, 01/12/2024 - 21:24

200X fern micronutrients

200X fern micronutrients kdorfman Fri, 01/12/2024 - 21:26

Fern medium from powder

Fern medium from powder kdorfman Wed, 01/24/2024 - 14:20

Carolina 156782

Mix contents of vial with 800 mL water

pH to 6 (NaOH)

Bring to 1 L

For single batch:

  • Add 10g Bacto Agar

  • Autoclave 45 min

For small batches:

  • Aliquot 167 mL into 6 200 mL media bottles

  • Add 1.67 g Bacto Agar to each bottle

  • Autoclave 30 min