Microscopes

Microscopes kdorfman Mon, 01/09/2012 - 15:20

Nikon Filter Cubes

label on filter wheel excitation wavelength emission wavelength used for label on cube
UV UV (360 nm) blue (460 nm) DAPI UV-2E-C
B B (480 nm) green (535 nm) GFP, fluorescein B-2E/C
G G ( 560 nm) red (630 nm) Texas red, rhodamine Y-2E/C
LP B (470 nm) long pass green - red (>500 nm) chlorophyll & GFP simultaneously B-2A
mOrange1 G (530 nm) orange (575). m orange Chroma 49014

Details:

Ultraviolet Excitation Filter Block UV-2E/C Specifications:

Excitation Filter Wavelengths: 340-380 nanometers (bandpass, 360 CWL)

Dichromatic Mirror Cut-on Wavelength: 400 nanometers (longpass, LP)

Barrier Filter Wavelengths: 435-485 nanometers (bandpass, 460 CWL)

Blue Excitation Filter Block B-2E/C Specifications:

Excitation Filter Wavelengths: 465-495 nanometers (bandpass, 480 CWL)

Dichromatic Mirror Cut-on Wavelength: 505 nanometers (longpass, LP)

Barrier Filter Wavelengths: 515-555 nanometers (bandpass, 535 CWL)

Yellow Excitation Filter Block Y-2E/C Specifications:

Excitation Filter Wavelengths: 540-580 nanometers (bandpass, 560 CWL)

Dichromatic Mirror Cut-on Wavelength: 595 nanometers (longpass, LP)

Barrier Filter Wavelengths: 600-660 nanometers (bandpass, 630 CWL)

Blue Excitation Filter Block B-2A Specifications:

Excitation Filter Wavelengths: 450-490 nanometers (bandpass, 470 CWL)

Dichromatic Mirror Cut-on Wavelength: 500 nanometers (longpass, LP)

Barrier Filter Wavelengths: 515 nanometer cut-on (longpass, LP)

mKO/mOrange Specifications:

Excitation Filter Wavelengths: 515-545 nm

Dichromatic Mirror cut-on wavelength: 550 nm

Barrier Filter Wavelengths: 555 - 595 nm

CoolLED manual


  1. installed on scope 7 for Akiko Okusu's class ↩︎

DIC

DIC bcrcstaff Tue, 11/20/2018 - 15:29

(On microscope 7)

Prism combinations

objective magnification prism on condenser turret prism on objective turret
Plan Fluor phase 10x N1 10x
Plan Fluor phase 40x (short working distance) N2 40x I
S Plan Fluor phase 40x (extra-long WD) N2 40x III
Plan Fluor phase 100x oil N2 100x II
Achromat w/iris brightfield 100x oil no DIC