Fish 2012

materials

  • no. 5 forceps

    • Fisher S95311 Straight; Ultra fine pointed tips; Aven Tools 18056ER $12.53
    • Digikey 243-1099-ND Aven Tools 18062-ER $15.65
    • Digikey 243-1054-ND Aven Tools 18062EZ $5.75 (green)
    • 243-1051-ND 18056EZ (turquoise) sharpest!
    • 243-1053-ND 18013EZ (yellow)
  • methyl cellulose 3% ~200 mL

    Flinn Scientific M0171 $7.55 100 mL

It takes ~ a week of stirring to make the powder ( Fisher S80080 100G) go into solution at this concentration!

  • Fish water (get a carboy full from Craig)

  • Lithium Chloride (MW = 42.4) solutions:

    • 10M LiCl in 10 mL H2O (4.24 g) (vortex in 15 mL conical. gets hot.) Check stock solutions first

    • 0.3 M LiCl in fish water (1.272 g/100 mL)

    1 ┬ÁL 10 M/1 mL fish water

  • deep depression slides (Pyrex 9-spot plates)

  • 60 mm petri dishes - CellTreat with gripping ring are good for this (sterility not necessary)

  • glass-bottom 29 mm petri dishes (InVitro Scientific)

  • 2 packs (2 x 48) Netwell baskets (Corning 3477)

Fisher 07-200-211 $215.55

  • 12 well plates (for the baskets to fit in)

  • timers

  • superglue

  • short tip pasteur pipettes

  • fishing line

  • transfer pipets (large, non-sterile)

  • mirror base dissecting microscope