Resistance 2013
Resistance 2013 kdorfman Thu, 01/17/2013 - 18:43Antibiotics 2013
Antibiotics 2013 kdorfman Mon, 01/28/2013 - 19:48(weight indicates how much should be on one disk.)
Calculate concentration from volume needed per disk (25 uL)
Make 35 mL of each (because the filter syringe only holds that much)
See recipes in stock solutions.
Make 35 mL each, make 48 0.7 mL aliquots
| Antibiotic | ug/disk | mg/mL | category | solubility | Fisher # |
|---|---|---|---|---|---|
| Ampicillin | 10 | 0.4 | beta-lactam | 50 mg/mL in 1 M NH4OH | |
| tetracycline | 30 | 1.2 | tetracycline | 10 mg/mL in water | |
| cipro | 5 | 0.2 | quinolone | 30 mg/mL in water | |
| Kanamycin | 30 | 1.2 | Aminoglycoside | 50 mg/mL in water |
cipro solubility http://www.inspiralis.com/go/antibiotics.php 30 mg/mL in water
ampicillin solubility 50MG/ML IN 1M AMMONIUM HYDROXIDE http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&sym… ampicillin sodium salt is soluble 50 mg/mL in water http://www.bioxys.com/i_gentaur/ampicillin_sodium.htm
Kanamycin 50 mg/mL in water
Tetracycline 10 mg/mL
The HCl forms are more water soluble
Plate Tally 2013
Plate Tally 2013 kdorfman Mon, 02/04/2013 - 15:03| type | poured | remaining | used |
|---|---|---|---|
| LB | 222 | 145 | 77 |
| McC | 1006 | 280 | 726 |
| M-H | 1150 |
Resistance 1 - 2013
Resistance 1 - 2013 kdorfman Thu, 01/17/2013 - 18:52Jan 29 & 31, 2013
200 LB plates
200 MacConkey plates
200 sterile swabs
Biohazard waste beaker at each table
Resistance 2 2013
Resistance 2 2013 kdorfman Mon, 02/04/2013 - 15:0120 MacConkey plates per student
sterile swabs
gloves
spray ethanol
markers
Resistance 3 2013
Resistance 3 2013 kdorfman Mon, 02/04/2013 - 15:1426 Mueller Hinton plates
sterile swabs
26 1-mL aliquots physiological saline
0.9% NaCl (=0.9 g/100 mL = 9 g/L)
sterile sticks
parafilm
markers
gloves
micropipettors & tips
vortex
antibiotic solutions in freezer
sterile disks
sterile forceps
rulers
microtube racks