Vasopressin for 6.2

Vasopressin for 6.2 kdorfman Mon, 11/04/2013 - 17:26

1 mg/mL (= 0.9225 mM) Vasopressin stock in water


1 µM dilution to make student solutions

1 µL 1 mg/mL in 922.5 µL water (NO CALCIUM, so stock can be used for Ca-free solutions )


10 nM (= 1.084 µg/mL) Vasopressin in HBS for students

Use Vasopressin aliquots from Lab 6.1


Calcium Free Vasopressin 10 nM

10 nM (= 1.084 µg/mL) for students

1 part 1µM dilution to 99 parts Ca-free HBS

Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 8 groups = 12 mL

Make 15 mL: 150 µL + 15 mL Ca-free HBS

Make 8 aliquots of 1.6 mL