Yeast 2015

Yeast 2015 kdorfman Fri, 01/16/2015 - 19:17

1/22 Th

  • slides,
  • coverslips
  • razor blades
  • scotch tape
  • valap
  • heat block (6)
  • microfuge (6 24 slot)
  • 50 mL conicals
  • 15 mL conicals
  • 200 mL beakers (1 per group)
  • Q-tips (long)

Tu 1/27/15

Tu 1/27/15 kdorfman Thu, 01/22/2015 - 16:40

*4 PCR machines with dual blocks - enough for eight groups of students

*8 DNA gel apparatus

*3 weighing balance for pipetting exercise

*10 mM dNTPs, 60 microliters total

*Phusion DNA polymerase, 10 microliters total (we prefer Phusion from NEB because we are amplifying with 80 nt long primers. I don’t recall if your freezer has any of this DNA polymerase. If not, I’m willing to just use the one in my lab)

For Thursday, Jan 29th:

MBOMS will run gel and need to coordinate with other labs to use the gel doc.