2.1: Counting bacteria

Counting Bacteria

Question of the day: What concentration has the stock culture?

Materials

  • Clean clear 96 well plate
  • Sterile glass beads
  • Sterile microfuge tubes
  • Ice buckets (fill with ice at beginning of period)
  • 8 LB plates per pair
  • ice cold LB broth
  • ~4 mL E. coli in stasis - ice cold
  • spray bottle of 70% EtOH to clean tables
  • hemocytometer

Program plate reader:

  • Bug_OD
  • A1-A12, B1-B12

Bio-hazard waste bin for

  • multi-well plates
  • agar plates after counting
  • little tubes of E. coli
  • hemocytometers