3.3: Direct fluor

Direct Fluorescent Labeling of Organelles


CHECK THIS FOR HBS vs PBS!!


Cells

3t3, LLCPk

  • Fixed in paraglut for phalloidin
    • 8 3t3 (can be fixed as late as the morning of lab)
    • 8 LLCPk (can be from pre-semester prep)
  • Live on small diameter MatTek dishes for Mitotracker and ceramide
    • 8 3t3
    • 8 LLCPk

Reagents

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ceramide.pdf160.64 KB