5.1 - 5.3 expression

5.1 Microarray resources M 4/2/12

MAKE SURE GENEVESTIGATOR ACCOUNT IS ACTIVE!!

5.2 Statistical analysis of microarray data W 4/4/12

Tell students what chemicals are available

  • ABA (2014)
  • ammonium nitrate
  • 1aminocyclopropanecarboxylic acid (converts to ethylene) (dry and frozen stock)
  • kinetin
  • giberellic acid
  • salicylic acid
  • IAA (dry and frozen stock) Indole-acetic acid
  • chitin (dry) (to mimic fungus attack)
  • lots of inorganic salts

http://www.phytotechlab.com/searchresult.aspx?categoryid=10

http://www.plantmedia.com/categories/3_43_288_691/Tissue-Culture-Hormone...

paper on germination in salt: http://www.researchgate.net/publication/255710805_The_effect_of_salt_str...

5.3 RT-PCR Planning M 4/9/12

  • 2-week old seedlings on plates - 2 plates per group (plates to refrigerator F 3/23, to growth chamber M 3/26)
  • 1-week old seedlings on plates 1 - 2 plates per group (for transfer experiments) (plates to refrigerator 3/30, to growth chamber 4/2)

  • 10 microfuge tubes with sterile, imbibed seeds in small batches for germination experiments (enough in each tube for one plate) (4/6)

  • 1 L MS in 100 mL aliquots for students to pour plates and add drugs or hormones, etc.)

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