Extraction prep

Equipment

  • razor blades
  • transilluminators
  • face shields
  • cutting boards
  • dry bath at 50C
  • columns & collection tubes

Solutions

  • Add ethanol to Buffer PE
  • Buffer QG ~2 mL (steps 4 & 8)
  • isopropyl - ~0.5 mL
  • NaOAc 3M, pH 5.0 ~25 µL
  • Buffer PE ~ 1.75 mL
  • Buffer EB ~ 75 µL (=Tris-Cl 10 mM pH 8.5)
  • 6x loading dye
  • 6 gels 1.5% (= 300 mL TAE + 4.5 g agarose + 3 µL EtBr)