Medium 21

Medium 21 kdorfman Wed, 12/15/2021 - 21:44

Mark with 2 red lines

Add 1 black line for carbenicillin (ampicillin)

Add 1 green line for IPTG

M21 from stocks

M21 from stocks kdorfman Wed, 12/15/2021 - 21:19
To make plates (100 mm)
(for a final volume of mL of medium)
start with: mL water
add: mL 1M K2HPO4
add: mL 1M KH2PO4
add: g Difco Bacto Peptone, and bring to final volume
add: g agar
autoclave for: minutes

Add 50 - 100 µg/mL ampicillin (carbenicillin) and 1 mM IPTG if needed

Medium 21 from dry

Medium 21 from dry kdorfman Tue, 12/14/2021 - 21:09
To make plates (100 mm)
you'll need: mL water
add: g K2HPO4
add: g KH2PO4
add: g peptone (Difco Bacto Peptone, not proteose)
add: g agar
autoclave for: minutes

If required, add IPTG 0.5mM (0.5 mL 1M per L medium) to 1 mM (1 mL 1M per L medium),

and ampicillin