Planting schedule 2022
Planting schedule 2022 kdorfman Thu, 12/23/2021 - 15:54Pots for students to plant seeds for genomic DNA
12 pots for Week 1 (1/26/22)
Planting for Week 5:
Arabidpsis essentials (2/23/22):
- 2 seeds in each of 6 rose pots every week,
- starting 1/12 (2 week before classes begin),
- for a total of 36 pots
- 6 1/2 MS plates 1 week before week 5 (seeds to fridge on 2/14/22)
Students plant mutant seeds:
- 12 groups, 2 pots mutants, 2 pots Col0
- for a total of 48 3" pots
Planting for Week 11:Gene expression planning:
Seeds on plates for RNA low and high iron experiments: ? at least 12 1/2 MS plates, 6 plates low iron.
5 seeds in each of 24 pots every week for 4 weeks, starting 6 weeks before week 11. (weeks 7, spring break, 8, and 9), for a total of 96 pots
1 row of seeds on each of 24 plates, 1 week before week 11
Date | plant | for |
---|---|---|
1/12 | 6 rose pots | 6 week old Arabidopsis |
1/19 | 6 rose pots | 5 week old Arabidopsis |
1/26 | 6 rose pots | 4 week old Arabidopsis |
2/02 | 6 rose pots | 3 week old Arabidopsis |
2/09 | 6 rose pots | 2 week old Arabidopsis |
2/16 | 6 1/2 MS plates | 1 week old Arabidopsis |
2/23 | ||
3/01 | 12 1/2 MS plates | seedlings on cellophane for cDNA (iron expt) |
3/03 | plates to growth chamber | start seedlings growing |
3/07 | 24 pots | 6 week old for gene expression |
3/11 | txfer plated seedlings | half to MS, half to low iron |
3/14 | harvest iron expt seedlings | Stavroula makes cDNA |
3/14 | 24 pots | 5 week old for gene expression |
3/21 | 24 pots | 4 week old for gene expression |
3/28 | 24 pots | 3 week old for gene expression |
4/06 | 24 plates | 2 week old for gene expression |
4/13 | 24 plates | 1 week old for gene expression |