Planting schedule 2022

Planting schedule 2022 kdorfman Thu, 12/23/2021 - 15:54

Pots for students to plant seeds for genomic DNA

12 pots for Week 1 (1/26/22)

Planting for Week 5:

Arabidpsis essentials (2/23/22):

  • 2 seeds in each of 6 rose pots every week,
  • starting 1/12 (2 week before classes begin),
  • for a total of 36 pots
  • 6 1/2 MS plates 1 week before week 5 (seeds to fridge on 2/14/22)

Students plant mutant seeds:

  • 12 groups, 2 pots mutants, 2 pots Col0
  • for a total of 48 3" pots

Planting for Week 11:Gene expression planning:

Seeds on plates for RNA low and high iron experiments: ? at least 12 1/2 MS plates, 6 plates low iron.

5 seeds in each of 24 pots every week for 4 weeks, starting 6 weeks before week 11. (weeks 7, spring break, 8, and 9), for a total of 96 pots

1 row of seeds on each of 24 plates, 1 week before week 11

Date plant for
1/12 6 rose pots 6 week old Arabidopsis
1/19 6 rose pots 5 week old Arabidopsis
1/26 6 rose pots 4 week old Arabidopsis
2/02 6 rose pots 3 week old Arabidopsis
2/09 6 rose pots 2 week old Arabidopsis
2/16 6 1/2 MS plates 1 week old Arabidopsis
2/23
3/01 12 1/2 MS plates seedlings on cellophane for cDNA (iron expt)
3/03 plates to growth chamber start seedlings growing
3/07 24 pots 6 week old for gene expression
3/11 txfer plated seedlings half to MS, half to low iron
3/14 harvest iron expt seedlings Stavroula makes cDNA
3/14 24 pots 5 week old for gene expression
3/21 24 pots 4 week old for gene expression
3/28 24 pots 3 week old for gene expression
4/06 24 plates 2 week old for gene expression
4/13 24 plates 1 week old for gene expression