6.4 RNA extraction 2022

RNA Extraction

Materials for the class:

• 6 centrifuges
• Tissuelyzer blocks in the -80
• LN2
• Freezer box to keep RNA in the -80

Materials per group:

• scissors
• ice bucket
• jeweler's scale
• 6 grinding tubes with 2 metal beads each
• 6 lilac columns
• 6 pink columns
• 6 capless collection tubes

Reagents per group:

• ~3 mL RLT + β-Mercaptoethanol
• 1.5 mL 95% Ethanol
• 4.2 mL buffer RW1
• 6 10 uL aliquots DNAse
• 420 uL buffer RDD
• 6 mL buffer RPE
• 300 uL RNase free water