Retinol kdorfman Wed, 04/27/2022 - 17:09

Fisher J62079

Store in freezer; hygroscopic


MW = 286.459

General description (from Fisher)

  • All-trans-retinol, commonly known as Vitamin A, is a fat-soluble essential nutrient
  • All-trans-retinol is involved in hormonal signaling via the retinoid receptors
  • It is an important regulator of cell division and apoptosis
  • Deficiency can lead to vision, bone, immune, and skin disorders

Practically insoluble aq. soln. or glycerol; soluble in absolute alcohol, methanol, chloroform,ether,fats and oils.

Can make a 50 mg/mL solution in 95% ethanol, but it's very hard to weigh

From Sigma information sheet:


  • RE is practically insoluble in water or glycerol. It is soluble in absolute ethanol, methanol, chloroform, ether, fats and oils.
  • RE has been dissolved at 50 mg/ml in chloroform; a clear yellow to orange solution results.
  • Stock solutions of RE (1 mg/ml) were prepared in ethanol, diluted in DMSO under low light conditions and stored at -50°C under nitrogen in brown glass vials.
  • RE solutions (50 μM) were sterile filtered before use.
  • RE both as a solid and in solution is readily oxidized in air and inactivated by UV light.
  • To reduce photodestruction of RE, manipulations of RE solutions can be performed under yellow or red light.
  • Solutions may be stabilized by dissolving in oil, by the addition of anti-oxidant compounds including a-tocopherol or hydroquinone or by conversion to the palmitate and acetate esters.
  • It is recommended to prepare solutions fresh for optimal quality. However, if absolutely necessary, store solutions in the dark under an inert atmosphere at least at -20°C preferably at -70°C
  • Solvents preferred for storage are peroxide-free ethyl ether, acid-free acetone or ethyl acetate.
  • For short term storage, ethanol is suitable as a solvent for spectroscopic analysis.


  • The isolation of retinol from human plasma has been described.
  • RE is an effective antioxidant displaying lipoperoxy radical scavenging activity.
  • The interactions between RE and Vitamin E (a-tocopherol) in suppressing lipid peroxidation were observed in bovine retinal membrane preparations.
  • RE may influence the production of transition vesicles by stimulating the activity of a protein disulfide isomerase-like activity involved in vesicle formation.
  • RE may be involved in immune system mechanisms; an RE deficiency will depress the immune response producing a negative effect on both humoral and cellular immunity.
  • (10 μM) and other retinoid compounds effectively induced sanguinarine and chelerythrine (benzophenanthridine alkaloids) accumulation in suspension-cell cultures of Sanguinaria canadensis in a way similar to fungal elicitation.
  • RE (10 μM) stimulated DNA synthesis and possibly repair mechanisms in Sertoli cells of rat.


The USP unit of vitamin A (same as the International Unit6) is equal to 0.3 μg of the pure all-trans isomer of retinol which is equivalent to 0.344 μg of all-trans retinyl acetate. RE and its metabolites, including retinoic acid, are part of the retinoid class of compounds, involved in vision, normal embryo morphogenesis and in the regulation of proliferation and differentiation of a number of cell types. Current information and hypotheses on the absorption, transport, storage and metabolism of this fat soluble Vitamin A (retinol) have been reviewed. Studies on RE metabolism including its mobilization and transport in plasma and in tissues via serum and cytosolic retinol-binding proteins have been described.