| 1 M Tris HCl pH 7.5 |
Isothermal mix |
|
no |
on buffer shelf |
| 1 M MgCl2 |
Isothermal mix |
|
no |
on buffer shelf |
| 10 mM dNTP |
isothermal mix |
Fisher R9012 |
no |
in RT reagents box (-20) |
| 1M DTT |
isothermal mix |
Acros 426380500 |
no |
in refrigerator door |
| Peg 8000 |
isothermal mix |
Fisher 04344322 |
yes |
above chem bench prep room |
| NAD |
isothermal mix |
MP Biomedicals 02100499.1 |
yes |
|
| T5 exonuclease |
PCR |
Fisher M0663S |
yes |
Maresca freezer box |
| Phusion polymerase |
PCR |
Fisher F530S |
yes |
Maresca freezer box -20 |
| Taq ligase |
PCR |
NEB M0208L |
yes |
Maresca freezer box |
| SiR Tubulin |
microtubule stain |
Fisher NC0958386 |
yes |
Maresca freezer box -20 |
| T7 RiboMAX Express RNAi System |
RNAi expts |
Promega PRP1700 |
yes |
Maresca freezer box |
| Heat inactivated FBS |
S2 culture |
Fisher 10082147 |
yes |
freezer -20 |
| Anti-anti |
S2 culture |
Fisher F530S |
no |
tissue culture aliquots freezer box |
| S2 medium |
S2 culture |
Fisher 21720-24 |
ordered 4 |
above fridge in tissue culture room |
| ZR plasmid miniprep |
S2 transformation |
D4015 |
yes |
above bench in prep room |