T7 RiboMAX
T7 RiboMAX kdorfman Wed, 09/21/2022 - 19:46Transcription mix
Students make the transcription mix from the kit
1 reaction per group
| Reagent | Sample reaction |
|---|---|
| RiboMAX™ Express T7 2X Buffer | 10 µL |
| linear DNA template (1µg total) | 1– 8µL |
| Nuclease-Free Water | 0–7µl |
| Enzyme Mix, T7 Express | 2 µL |
| Final Volume | 20 µL |