Master mix Master mix kdorfman Mon, 06/12/2023 - 15:05 To make Qiagen Master Mix + primers for studentsand reactions per studentat uL per reactionStart with: uL Qiagen Master Mixadd: uL forward eGFP primer (100 mM)add: uL reverse eGFP primer (100mM)add: uL forward Hedgehog Inhibitory Protein primer (100 mM)add: uL reverse Hedgehog Inhibitory Protein primer (100 mM)add: uL waterfor a final volume of: uL Qiagen Master Mix plus primersaliquot: uL Qiagen Master Mix plus primers per student Master Mix, multiple primers Master Mix, multiple primers kdorfman Mon, 07/01/2024 - 22:10 NOT SURE WHY THIS DOESN"T CALCULATE To make Qiagen Master Mix + primers for students (or pairs of students)and reactions per student (or pair)and primer sets (R + F)at uL per reactionStart with: uL Qiagen Master Mixadd: uL of each F and R 100 uM primeradd: uL waterfor a final volume of: uL Qiagen Master Mix (including primers!)aliquot: uL Qiagen Master Mix (including primers!) per student (or pair)
Master Mix, multiple primers Master Mix, multiple primers kdorfman Mon, 07/01/2024 - 22:10 NOT SURE WHY THIS DOESN"T CALCULATE To make Qiagen Master Mix + primers for students (or pairs of students)and reactions per student (or pair)and primer sets (R + F)at uL per reactionStart with: uL Qiagen Master Mixadd: uL of each F and R 100 uM primeradd: uL waterfor a final volume of: uL Qiagen Master Mix (including primers!)aliquot: uL Qiagen Master Mix (including primers!) per student (or pair)