Bradykinin

1mg/mL Bradykinin stock
(= 1.0602 mM) Sigma B3259 - 1 mg septum bottle
MW = 106.02
Add 1 mL H2O to the 1 mg in the bottle (NO CALCIUM! - some will be used for the calcium experiments in Lab 7.2)

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2 µM Bradykinin in HBS

Use bradykinin aliquots from Lab 7.1

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2 µM Bradykinin, Calcium-free
(=2.12 µg/mL)

Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 7 groups = 10.5 mL

Frozen aliquots are 21.2 µL 1 mg/mL. Makes 10 mL

~2 µL 1 mg/mL stock per 1 mL working solution

21.2 µL 1 mg/mL stock in 10 mL Ca-free HBS

Make 6 aliquots of 1.6 mL

Make more if necessary. (Aliquots should have been enough for 15 mL?)