Week 09 PCR MC1R

Week 09 PCR MC1R kdorfman Mon, 07/24/2023 - 20:25

Dog DNA labs, week of 10/30

students set up 2 identical rxns for MC1R per pair

plus 1 tube each for ASIP, Def103, TyRP1

Equipment

  • Nanodrop (for students who didn't finish last week)
  • Thermocycler (program: ???)

Reagents

Instructors make MasterMix + primers; students add 100 ng DNA plus water to make ?? uL

  • Qiagen MasterMix
  • forward primer
  • reverse primer
  • sterile water

Paper activities

  • Complete genetics of dog hair packet
  • Complete MC1R activity
  • Protein/structure/function activity