Week 11 Restriction digest

Dog DNA labs, week of 11/13

Genotyping loci B (TyRP1), E (MC1R), K(defb103)

We provide folder with sequences

Restriction digest TyRP1 ("B" locus) 1 uL/rxn each:

• Aci1
• Mnl1

We provide uncut

Bioinformatics

• 4peaks to clean sequences Ma, Mb, Mc
• CAP3 to assemble contigs to get MC1R sequence
• Clustal also!
• BLAST to compare to known nt sequences: MC1R (E), Defb103 (K)
• Expasy (nucleotide to amino acid sequence)
• Clustal omega to align aa to known sequences