Week 04
Week 04 kdorfman Mon, 07/24/2023 - 20:50Yeast Labs week of 9/25/23
(See also seedling observations)
Pipetting exercises:
- Colorimetric pipetting exercise
- Weighing water
- 1 balance per pair
- weigh boat
- beaker of dH2O
- extra batteries
DNA extraction from yeast
- Reagents
- 200 mM LiOAc, 1% SDS (4x 100 uL/pair)
- 95% EtOH (4 X 300 uL/pair)
- 70% EtOH ( 4 X 400 uL/pair)
- TE ( 4 x 80 uL/pair)
- Equipment
- microfuge tubes
- hot block at 70C
- centrifuges for 1.5 mL tubes, 15,000 x g
- paper towels
- Nanodrop
Sequences
- send out for sequencing?
- 4 strains per table
- really? or pull sequences out of her back pocket?
Check MV replica plate from last week
- check diploids
- draw picture
- label
- transfer
- some??? to YED (to check the next day, next day toothpick to YEKAc plate 1/pair (=48)
- pick one to YED (class "pre-spore")
YED ?2 per pair? = 48
YEKAc??