McCoy's 5A

McCoy's 5A (modified) (Fisher 16600-082)

For HCT116 cells (a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases. In vitro, HCT116 cells grow with a doubling time of about 18 hours.)

Drew's recipe:

10% FBS, 1% Pen-strep

• 500 mL McCoy
• 55 mL FBS
• 5.5 mL pen-strep

OR:

• remove 55 mL from the 500 mL McCoy's from Fisher
• add 50 mL FBS
• add 5 mL pen strep (e.g., MP 1670246)
• resterilize

Filter sterilize

If you have	mL McCoy's
mix with	mL FBS
and	mL Pen-Strep
for a final volume of	mL McCoy's ready to use