10/17/23

Trial run for Lamin antibodies

Fix 15 min in paraglut

1^o Ab: Rabbit anti-lamin B

• dilute 1:1000 with PBS-Tw-Az-BSA
• 2 µL antibody into 2 mL buffer
• 1 hr 37C

2^o Ab: goat anti-rabbit

• 1 hr RT incubation

Cell type	# coverslips
MEF WT NLS-GFP (40)	3
HCT-116 WT (60)	3
DU-145 (74)	4

Stain with Hoechst (1:10,000) in the final PBS-Tw-Az rinse

Mount with non-DAPI Fluoromount (Fisher OB100-01)