10/24/23

10/24/23 kdorfman Mon, 10/16/2023 - 19:36

Students begin projects

Kate provides fixed coverslips (seeded 10/22, fixed 10/24/23)

Cell line medium variant treatment no. coverslips
HCT116 McCoy WT (60) 4
LB1-AID-GFP (68) 2
MEF DMEM WT (39) control 6
VPA
DZNP
LMNB1-/- (02) 3
DMEM+G418 LA KD (37) 3
Prostate cancer RPMI-1640 DU145 (74) 2 +3 from Mai
RPMI-1640 LNCaP (77) 2 + 3 from Mai
DMEM PC3 (76) 2

Materials:

Project people scope IF coverslips IF details
MEF VPA Olivia & Juliana 1 1x 39 unt, 1X 39 VPA, another VPA if pos standard NP LB1 IF
MEF DZNep Karan & Nate 2 1x 39 unt, 1X 39 DZNep, another if pos standard NP LB1 IF
MEF LMNB1-/- Cole & Grif 3 1X 39, 2X 02 LMNB1-/- 39 & 02 NP LB2 IF, 1x 02 NP LB1 IF
MEF LA KD Shrushti, Emma, Anna 8 1X 39, 2X 39 standard NP LB1 IF
HCT116 LB1 live 1 Sam & Jillian 5 68, 1x unt & 1x VPA LB2 IF only
HCT116 LB1 live 2 Isabelle & Jason 6 60, 1x unt 1x VPA; 60, 1x unt 1x VPA one set NP LB1 IF; other set NP LB2 IF
Prostate Cancer 1 Tyian & Antonia 4 2x 74, 1x 76 standard NP LB1 IF
Prostate Cancer 2 Kelsey & Pedro 7 2x 74, 1x 77 standard NP LB1 IF