Marine Biology
Marine Biology kdorfman Mon, 01/15/2024 - 17:322024 Fall Marine Bio
2024 Fall Marine Bio kdorfman Tue, 10/08/2024 - 16:23For Friday 10/11
Growth chamber spawning program
Growth chamber spawning program kdorfman Fri, 05/17/2024 - 19:17Basic Parameters:
Light from 9 pm Sunday to 1 pm Monday, otherwise dark
Warm from 9 pm Sunday til 9 am Monday, otherwise cool
Day | time | temp | light level |
---|---|---|---|
M | 13:00 | 18 | 0 |
Sun | 21:00 | 25 | 3 |
M | 9:00 | 18 | 3 |
M | 13:00 | 18 | 0 |
Schedule 3 programs, like this:
Program 1, repeat 6 times:
Day | time | temp | light level |
---|---|---|---|
M | 00:00 | 18 | 0 |
Program 2 (spawning), do once, following 6x Program 1
Day | time | temp | light level |
---|---|---|---|
Sun | 00:00 | 18 | 0 |
21:00 | 251 | 3 |
Program 3, do once
Day | time | temp | light level |
---|---|---|---|
M | 00:00 | 25 | 3 |
9:00 | 18 | 3 | |
13:00 | 18 | 0 |
Return to Program 1
-
There is a temperature spike when the light comes on, so leave the temp setting at 18 for an hour (until 22:00), then raise it to 25. ↩︎
Sea Water for 424
Sea Water for 424 kdorfman Mon, 01/15/2024 - 18:142 carboys SeaWater (31-35ppt salinity):
- 31-35g InstantOcean Reef Salt in 1 L
- Submerge a bubbler or a pump so that it is constantly being mixed
Nematostella SW is 1/3 ASW
F/2 Medium for algae (424)
F/2 Medium for algae (424) kdorfman Mon, 01/15/2024 - 18:165 liters of f/2 medium for algae.
L1 Medium Kit from Bigelow NCMA contains the nitrate, phosphate, trace minerals, and vitamins. Add the antibiotics from prep room freezer stocks.
Re-inoculate once during week of 1/15
TA & Students will re-inoculate once monthly
50 mL/month x 4 months x 16 students = 3200 mL
Make 4 L for the semester.
Plus ASW to final volume
Filter sterilize
Trace minerals for F/2
Trace minerals for F/2 kdorfman Mon, 06/24/2024 - 15:46Buy L1 Medium Kit from Bigelow NCMA
Replacement trace minerals solution
Component | Concentration in final medium |
---|---|
Na2EDTA · 2H2O | 1.17 x 10-5 M |
FeCl3 · 6H2O | 1.17 x 10-5 M |
MnCl2·4 H2O | 9.00 x 10-7 M |
ZnSO4 · 7H2O | 8.00 x 10-8 M |
CoCl2 · 6H2O | 5.00 x 10-8 M |
CuSO4 · 5H2O | 1.00 x 10-8 M |
Na2MoO4 · 2H2O | 8.22 x 10-8 M |
H2SeO3 | 1.00 x 10-8 M |
NiSO4 | 1.00 x 10-8 M |
Na3VO4 | 1.00 x 10-8 M |
K2CrO4 | 1.00 x 10-8 M |
Vitamins for F/2
Vitamins for F/2 kdorfman Mon, 06/24/2024 - 15:51Buy L1 Medium Kit from Bigelow NCMA
Replacement Vitamin Stock Solution
Component | Concentration in Final Medium |
---|---|
thiamine · HCl (vit. B1) | 2.96 x 10-7 M |
biotin (vit. H) | 2.05 x 10-9 M |
cyanocobalamin (vit. B12) | 3.69 x 10-10 M |