HeLa for 1st day

HeLa for 1st day kdorfman Wed, 08/28/2024 - 20:33

For "Learn your Microscope" 9/3

9 HeLa (Vikash) coverslips

Fixed in paraglut

Stained for alpha tubulin:

  • rat anti tubulin (directions on tube)
  • goat anti rat TRITC (1:1000)

Stained for green actin

Mounted in DAPI

Note: Red tubulin was gorgeous! Let's see if it lasts longer than the FITC.