Media for 5.2
Media for 5.2 kdorfman Mon, 11/14/2011 - 17:19Enough to grow overnight cultures and make dilute early log phase cultures of all 7 chemotaxis strains for use during lab.
Swarm Agar
| ingredient | 750 | 1000 | 1500 | mL |
|---|---|---|---|---|
| tryptone | 7.5 | 10 | 15 | g |
| NaCl | 3.75 | 5 | 7.5 | g |
| bacto-agar | 2.25 | 3 | 4.5 | g |
Mix tryptone and NaCl in water first.
Bring to final volume.
Divide into volumes suitable for the vessel(s) going into the autoclave. (E.g., 750 mL per 1 Liter bottle.)
Add correct amount of agar (3 g per L) to each container. Leave stir bar in container!
Autoclave 60 min
Sit on stir plate, stirring, until handleable
Pour 25 mL per plate. Plates do not keep well, so pour them the morning of the day they will be inoculated.
4 plates per group. (= 48 minimum) So make 1500 mL. Really need 55 plates + some tiny petri dishes to demonstrate swarm agar consistency.
Inoculate at 7 pm: 13 WT plates + 7 of each of the other strains.