5.6 RT-PCR analysis5.6 RT-PCR analysis margaret Wed, 11/16/2011 - 18:37
Make enough for 2 gels/pair 1.5% agarose
|gels||mL TAE||g agarose||µL EtBr|
Use EtBr TAE
Use LMW mass ruler
Prepare 15 μL of each of your PCR reactions for loading on gels by transferring to a clean, labeled 1.5 mL microfuge tube, and adding 3 μL of loading dye. Load the entire 18 μL – it is important that all lanes contain the same amount.
Load gels, and run at 100V for 45 minutes. Remember to load 5 μL (= 220 ng) of LMW Mass Ladder as your standard.