Pipettes and tips

Pipettes and tips kdorfman Thu, 06/18/2009 - 16:04

Classroom pipettes

Classroom pipettes kdorfman Thu, 01/03/2013 - 17:41

The pipettes in use in the ISB are Rainin micropipettors :

Pipet-Lite® with LTS®, which takes LTS cylindrical tips, and has red, green, or blue labels on the plunger

LTS PIPETTES (rooms 260, 262A, 268, 360, 364, 366A, 368)

Plunger	max uL	part #
Blue	1000	Rainin GPS-LTS 1000uL tips (30389292), in GPR-L1000 (blue) racks
Green	200	Rainin GPS-LTS 250 uL tips (30389299), in GPR-L250 (green) racks
Red	20	Rainin GPS-LTS 20uL tips (30389291), in GPR-L10 (red) racks

Colorimetric Pipetting Exercise kdorfman Wed, 06/21/2023 - 18:16

Mix 200 mM mono- (acid) and di-basic sodium phosphate (base) with bromothymol blue to get a range of colors from yellow to blue.

Put a 5 uL drop of bromothymol blue in each square, then add the given amounts of sodium phosphate

pH	6.0	6.2	6.4	6.6	6.8	7.0	7.2	7.4	7.6	7.8
Na2HPO4	2.5	3.7	5.3	7.5	9.8	12.2	14.4	16.2	17.4	18.3
NaH2PO4	17.5	16.3	14.7	12.5	10.2	7.8	5.6	3.8	2.6	1.7

Per exercise, need:

So aliquot

Eppendorf Repeater kdorfman Mon, 09/04/2023 - 20:17

Dial	# aliquots	0.1 mL	0.2 mL	0.5 mL	1.0 mL	2.5 mL	5.0 mL	10 mL	25 mL	50 mL
		white	lt blue	purple	yellow	green	blue	orange	red	gray
		µL	µL	µL	µL	µL	µL	mL	mL	mL
*	100	1	2	5	10	25	50	0.1	0.25	0.5
1	50	2	4	10	20	50	100	0.2	0.50	1
*	33	3	6	15	30	75	150	0.3	0.75	1.5
2	25	4	8	20	40	100	200	0.4	1.00	2
*	20	5	10	25	50	125	250	0.5	1.25	2.5
3	16	6	12	30	60	150	300	0.6	1.50	3
*	14	7	14	35	70	175	350	0.7	1.75	3.5
4	12	8	16	40	80	200	400	0.8	2.00	4
*	11	9	18	45	90	225	450	0.9	2.25	4.5
5	10	10	20	50	100	250	500	1	2.50	5
*	9	11	22	55	110	275	550	1.1	2.75	5.5
6	8	12	24	60	120	300	600	1.2	3.00	6
*	7	13	26	65	130	325	650	1.3	3.25	6.5
7	7	14	28	70	140	350	700	1.4	3.50	7
*	6	15	30	75	150	375	750	1.5	3.75	7.5
8	6	16	32	80	160	400	800	1.6	4.00	8
*	5	17	34	85	170	425	850	1.7	4.25	8.5
9	5	18	36	90	180	450	900	1.8	4.50	9
*	5	19	38	95	190	475	950	1.9	4.75	9.5
10	5	20	40	100	200	500	1000	2	5.00	10