3.5 Salk genotypes3.5 Salk genotypes margaret Wed, 11/16/2011 - 18:39
Identifying genotypes of Salk-line plants: Analysis
Goal for this lab: Determine the genotypes of Salk line plants to identify homozygous mutant individuals. Run PCR reactions from 3.4 on gels
Students will have 28 + samples, so they need two gels.
- 2 2% agarose gels per pair
- 1× TAE running buffer (no EtBr)
- Loading dye (2µL/lane)
- Fermentas MassRulerTM DNA ladder, low range (5µL/gel)