Latrunculin B
Latrunculin B kdorfman Wed, 11/30/2011 - 18:21Alexis Biochemicals 350-036-C100 Catalog # t110
Inhibits actin polymerization and disrupts microfilament organization, as well as microfilament-mediated processes
Reported to be 10 to 100-fold more potent than the cytochalasins. May act more slowly, though. Lots of variation between cell lines.
Inactivated by FBS. (Rinse medium off before treatment.)
MW = 395.5
Soluble in DMSO and ethanol.
Store in freezer.
Active concentration range: 90 nm ( =~0.1µM) to 2.5 µM
(2.5 µM = 25 x 100 nm)
Stock is 1mm
| µL 1mm stock | µL serum-free buffer or medium | µM final concentration |
|---|---|---|
| 1 * | 99 | 10 |
| 1 | 399 | 2.5 |
| 1 | 999 | 1 |
*Use this to make further dilutions