Cells for 3.2 Cells for 3.2 kdorfman Tue, 12/06/2011 - 15:01 Immunofluorescence Labeling Fixed cells on coverslips Probably should be fixed in formaldehyde, since the glutaraldehyde in paraglut interferes with the anti-Golgi staining. LLCPK - at least 2 per group Should be already in the refrigerator from pre-semester prep, in coplin jars of PBS-Tw-Az. Live cells For fixation with ?paraglut? formaldehyde? LLCPK - 1 per group plus extras (8) Plate lightly on coverslips 2 days before lab, or very lightly 3 days before.