DEB

DEB kdorfman Mon, 12/19/2011 - 17:40

DNA Extraction Buffer for Gene & Genome

  • 100 mM NaCl
  • 50 mM Tris, pH8
  • 25 mM EDTA
  • 1% SDS

Make from stock solutions

To make mL DNA Extraction Buffer
add: mL 5M NaCl
add: mL 1M Tris, pH 8
add: mL EDTA 0.5M
add: mL 20% SDS
add: uL BMe AT THE LAST MINUTE!