Solutions

Solutions to have at the beginning of the semester

Amount		conc	solution
100	mL	10%	Tween in water
50	mL	10%	Tween in PBS
100	mL	10%	Triton-X in water
100	mL	10%	Triton-X in PBS
5	L	10X	PBS
1	L	1x	PBS in 10 mL sterile aliquots
4	L	1x	PBS-Tw-Az
25	mL	10%	Sodium azide
250	mL	10X	HBS
100	mL	10 X	Ca-free HBS
500	mL	1x	Non-CO2 medium with serum
1000	mL	1x	Non-CO2 serum free medium
2	L	1x	F10-Hams
250	mL	1x	serum free F-10 Hams
1	L	1x	DMEM
20	mL		DAPI Fluoromount Fisher OB010020 2 bottles to share.