Anti-Fungal

Stock solution: 10 mg/mL in DMSO (insoluble in ethanol)

Store at 4C short term, -20 long term (in 15 mL tube, wrapped in foil with tape label)

Keep in the dark.

Final concentration in medium = 10 ug/mL

Add 1 mL per L

Freezes in the refrigerator.

Protect from light.

50 mg/mL stock

(Final concentration in medium = 50 µg/mL)

Lasts up to 4 weeks in poured agar at 4C

0.5 g in 10 mL water

filter sterilize

1 mL aliquots (enough for 1 L medium)

0.4 mg/mL for QSB

Make 35 mL: 0.28 mL 50 mg/mL stock

35 mL sterile water

(filter sterilize if any doubt about sterility)

48 0.7 mL aliquots

Can also use carbenicillin

to make:	mL amp solution
at this concentration:	mg/mL
add:	g dry ampicillin

(More stable than ampicillin)

Fisher 50841234b 25 mL Teknova C2130

100 mg/mL stock

effective concentration 50 - 100 µg/mL in medium

Freeze aliquots.

MP 19032

store dry at RT

Lasts up to 4 weeks in poured agar at 4C

Ciprofloxacin 10 mg/mL stock

(final concentration in medium = 10 µg/mL)

100 mg in 10 mL dilute acid (add HCl drop by drop, mixing in between till it dissolves)

filter sterilize

1 mL aliquots

freeze

0.2 mg/mL for QSB

0.7 mL 10 mg/mL stock

35 mL water

filter sterilize

48 0.7 mL aliquots

To make	mL 0.2 mg/mL cipro
you'll need:	g cipro

freeze

Tetracycline category

solubility 50 mg/mL in water

disk has 30 ug

solubility

50 mg/mL in ethanol

lasts up to 4 weeks in poured agar at 4C

10 mg/mL stock

(10 µg/mL in medium)

0.25 g in 25 mL EtOH

1 mL aliquots

freeze

0.6 mg/mL for QSB

2.1 mL 10 mg/mL stock

33 mL EtOH¹

48 0.7 mL aliquots

freeze

50 mg/mL stock (50 µg/mL in medium)

Concentration in poured agar drops to ~80% in 1 week, but stays stable for 3 more weeks at 4C

1.25 g in 25 mL H2O

filter sterilize

mL aliquots

freeze

1.2 mg/mL for QSB

0.84 mL 50 mg/mL stock

34.16 mL water

filter sterilize (if any doubt about sterility)

48 0.7 mL aliquots

To make	mL 1.2 mg/mL kanamycin
you'll need:	g kanamycin

freeze

In refrigerator in 362A

Fisher Streptomycin sulfate 50g

BP910-50 (discontinued)

MW 1457.58

~50 mg/L for agar

Standard is 10 mg/mL stock

Stable for 2 weeks in poured agar at 4C; 75% after 4 weeks

15 mg/mL stock

0.275 g tetracycline in 25 mL EtOH

1 mL aliquots

freeze

1.2 mg/mL for QSB

2.8 mL 15 mg/mL stock in 32.2 mL EtOH¹

48 0.7 mL aliquots

freeze

To make	mL 1.2 mg/mL tetracylcine
you'll need:	g tetracycline

Irgasan

Sigma 72779-5g-f

MW 289.54