Mussel saline
Mussel saline kdorfman Mon, 01/16/2012 - 15:06Mussel Ringers
from http://www.biolbull.org/cgi/reprint/181/1/175.pdf
- 5 mM NaCl
- 0.5 mM KCl
- 5 mM CaCl2
- 5 mM NaHCO3
- 5 mM Na2SO4
1X Ringers from 10X stock
100 mL 10X stock per L final volume
Ringers needed for
- diluting drugs
- keeping dissected mussels moist
10X Ringers from stock solutions
| Stock solution | ci (M) | cf (M) | 50 | 75 | 100 | 200 | final vol (mL) |
|---|---|---|---|---|---|---|---|
| NaCl | 5 | 0.05 | 0.5 | 0.75 | 1 | 2 | mL |
| KCl | 1 | 0.005 | 0.25 | 0.375 | 0.5 | 1 | mL |
| CaCl2 | 1 | 0.05 | 2.5 | 3.75 | 5 | 10 | mL |
| Na2SO4 | 1 | 0.05 | 2.5 | 3.75 | 5 | 10 | mL |
| NaHCO3 1 | 1 | 0.05 | 2.5 | 3.75 | 5 | 10 | mL |
| OR dry NaHCO3 | MW=84 | 0.05 | 2.1 | 3.15 | 4.2 | 8.4 | g |
pH 7.8
Filter sterilize (NaHCO3 may not be stable during autoclaving)
-
make fresh - it doesn't keep. ↩︎