Fisher AC159170050 5 gr, $13.92

MW = 181.7

Working concentration = 10^-4M = 0.01817 g/L

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10 mL 100X stock

0.01817 g acetylcholine

10 mL ringers or seawater

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12 mL 10X stock for students to make dilutions

1.2 mL 100X stock

Ringers or sea water to 12 mL

Aliquot 2 mL into 5 tubes

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15 mL 1X working concentration

0.15 mL 100X stock

Ringers or sea water to 15 mL

aliquot 1 mL into 14 tubes

(Fisher A550328M050 50 mg, $34.32)

MW = 212.68

soluble up to 100 mg/mL in water

Working Concentration = 10^-5M

X	M	mM	µM	mg/mL
1X	10^-5	0.01	10	0.0021268
10X	10^-4	0.1	100	0.021268
100X	10^-3	1	1000	0.21268
1000X	10^-2	10		2.1268

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1.4 mL 1000X stock

=10 mM

3 mg (use the mg scale in 266A)

1.41 mL 1X ringers or sea water

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12 mL 10X solution for students to make dilutions

0.12 mL 1000X stock

11.88 mL 1X Ringers or sea water

aliquot 2 mL into 5 tubes

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15 mL 1X working concentration for student use

0.015 mL 1000x stock

15 mL Ringers or sea water

aliquot 1 mL into 14 tubes