Carolina cultivation
Carolina cultivation kdorfman Thu, 02/16/2012 - 14:36Amoebae from Carolina:
131324 Chaos (Pelomyxa) carolinensis, Living $7.30
Order 12 dishes, 1 per group
Culture includes P. multimicronucleatum and other much smaller organisms, as well as the occasional rotifer.
Try washing, as per Tan:
Transfer individual amoebae with sterile transfer pipette to culture dish with Chaos saline
Leave at 22C in the dark overnight
Next day, they don't look any better (floating, not attached, few pseudopodia)
Feed them anyway.
No improvement.
Better to leave them in the Carolina dish.