Parameciavap

Parameciavap kdorfman Thu, 02/16/2012 - 16:25

Culturing Paramecia ala Parameciavap

PC - 250 mL Paramecia Culture 3000/mL in diluted Tubingen E3 medium for zebrafish embryos

PCM 100X Paramecium Culture Medium concentrate (250 mL)

PF - 200X Paramecium Food for high density cultures (50 mL)

Per Liter:

  • PCM 10 mL
  • H2O 1 L
  • PF 5 mL (can be added after initial incubation)
  • PC 33 mL

Keep at 25-27C

To make mL PCM + Food
add: mL PCM
add: mL PF
add: mL PC

After 1 week, add 5 mL PF/L

Culture 1 more week

[For zebrafish, filter and resuspend in Tubingen E3 (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, and 0.33 mM MgSO4)]