P.m. (KD)
P.m. (KD) kdorfman Sat, 02/18/2012 - 15:52Paramecia multimicronucleatum from Parameciavap
http://parameciavap.com/Paramecia.aspx ($45/250 mL)
Get PO from Charlene
To increase population size:
Test cultures for rotifers. Split flasks that have only paramecia
To prepare for student use:
- Sterile Erlenmyers with foam plug
- Chaos saline (http://wahoo.nsm.umass.edu/content/chaos-saline) to volume that gives maximum surface area/volume ratio)
Add sterile wheat seed (~1/25 mL)1
Transfer P.m. from PC to Chaos saline + sterile wheat seed (~1:100)
- Store at 22C in the dark
- Transfer ~10 mL culture to tissue culture flask with new seed (1 flask per pair of students)
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Wheat seeds support dense rotifer populations. Start the Paramecium culture only with cultures that have no rotifers. Take multiple samples to determine rotifer content. ↩︎