Gels
Gels kdorfman Thu, 05/24/2012 - 17:17EtBr gel
EtBr gel kdorfman Tue, 09/20/2022 - 15:02Ethidium bromide gel
*Add weighed agarose to measured TAE in a flask (about half the maximum volume of the flask)
*Boil in the microwave CAREFULLY (power level 0.5) until completely dissolved (check by swirling) DO NOT LET IT BOIL OVER
*Allow to cool slightly
*Add EtBr (in the fume hood)
*Aliquot into 50 mL conical tubes
*Put in rack in 65C waterbath
Clean-up
Rinse gel rigs within an inch of their lives
Put gels into zip lock bag, stick a hazardous waste sticker on it, schedule hazardous waste pickup (sign into CEMS)
pour used buffer into gallon jug with EtBr removing "tea bag", stir overnight, then pour down the drain
Gel Rigs
Gel Rigs kdorfman Wed, 01/11/2023 - 20:25| brand | room | L x W (cm) | gel vol | # small wells | small well uL | # large wells | large well uL |
|---|---|---|---|---|---|---|---|
| IBI | 264 | 10 x 7 cm | 50 mL | 15 | 25 | 8 | 50 |
| BioRad | 360, 364, 368 | 10 x 7 cm | 50 mL | 15 | 25 | 8 | 50 |
| Labnet | genetics | 10 x 15 cm | 100 mL | 20 | 30 | 16 | 50 |
Sybr Safe gel
Sybr Safe gel kdorfman Tue, 09/20/2022 - 15:011 uL /10 mL gel
*Add weighed agarose to measured TAE in a flask (about half the maximum volume of the flask)
*Boil in the microwave CAREFULLY (power level 0.5) until completely dissolved (check by swirling) DO NOT LET IT BOIL OVER
*Allow to cool slightly
*Add SYBR-safe
*Aliquot into 50 mL conical tubes
*Put in rack in 65C waterbath
Clean-up
Everything can go in the trash or down the drain