Fish 2012
Fish 2012 kdorfman Thu, 09/13/2012 - 15:00materials
materials kdorfman Thu, 09/13/2012 - 15:03- no. 5 forceps
- Fisher S95311 Straight; Ultra fine pointed tips; Aven Tools 18056ER $12.53
- Digikey 243-1099-ND Aven Tools 18062-ER $15.65
- Digikey 243-1054-ND Aven Tools 18062EZ $5.75 (green)
- 243-1051-ND 18056EZ (turquoise) sharpest!
- 243-1053-ND 18013EZ (yellow)
- methyl cellulose 3% ~200 mL > Flinn Scientific M0171 $7.55 100 mL
It takes ~ a week of stirring to make the powder ( Fisher S80080 100G) go into solution at this concentration!
Fish water (get a carboy full from Craig)
Lithium Chloride (MW = 42.4) solutions:
10M LiCl in 10 mL H2O (4.24 g) (vortex in 15 mL conical. gets hot.) Check stock solutions first
0.3 M LiCl in fish water (1.272 g/100 mL)
1 µL 10 M/1 mL fish water
deep depression slides (Pyrex 9-spot plates)
60 mm petri dishes - CellTreat with gripping ring are good for this (sterility not necessary)
glass-bottom 29 mm petri dishes (InVitro Scientific)
2 packs (2 x 48) Netwell baskets (Corning 3477)
Fisher 07-200-211 $215.55
12 well plates (for the baskets to fit in)
timers
superglue
short tip pasteur pipettes
fishing line
transfer pipets (large, non-sterile)
mirror base dissecting microscope