Grind tissue

Grind roots +/-, shoots +/- in ball mill (Sam's Retch Mixer Mill MM400)

• Precool the white block for the ball mill

• prepare 2 2-mL tubes per plate (label tubes on side as well as top)

  • 3 roots + iron
  • 3 roots - iron
  • 3 shoots + iron
  • 3 shoots - iron

• 2 metal beads per tube 3.2mm diameter ss beads (90g) - Cat. No. 11079132ss from www.biospec.com (Can also get from Fisher)

• cool in LN2

• Add tissue to cold tube, put back in LN2

• Put all tubes in cold block

• Shake 20 sec, check. The heat of shaking may start to defrost the tissue. Throw it back in the LN2. (Shake too long, and the cap may disintegrate!

• Return to LN2