PrimersPrimers kdorfman Fri, 05/31/2013 - 17:35
Primers come lyophilized.
Tube label says how many nmol in the tube (usually ~100-500)
Multiply nmol x 10 = μL of sterile water to add to make 100 μM stock. Fisher primer calculator
This is the only stock!
Put it away safely on instructor shelf in freezer.
Make a working stock for students (give them all of it):
- 12.5 μM (12.5 μL of the 100 μM stock + 87.5 μL water).
(If using the repeater pipet for the water, 85 µL water + 12.14 µL 100 µM stock)