E coli 2013
E coli 2013 kdorfman Wed, 09/11/2013 - 16:15Week before lab:
streak out control cells on agar plate
grow overnight at 37C
cover plate and refrigerate
Two days before lab:
pick colony, put into enough LB for all students (~250 mL)
grow on shaker overnight
Day before lab:
put LB in fridge
- need ~15 mL
- 2013 - have lots of 25 mL tubes of LB. Use them up
check during day for stasis (OD = ~2.6 in previous years; growth seems to stop at OD = ~2, which is still in the linear range)
NOTE: If left too long on shaker, get too many in chains. Check culture first thing in the morning with microscope. OD = 2, still swimming. Put in fridge.
refrigerate to stop growth
Dilute or spin down and resuspend to OD = ~2.5 (around max linear correlation to conc) with ice cold LB
Aliquot ~4 mL/tube
1 tube per pair