3.3 2013
3.3 2013 kdorfman Thu, 09/26/2013 - 14:40M 9/30
Labeling Cellular Structures
Cells
paraglut fixed LL's for students to stain for tubulin (make extra!)
paraglut fixed LL's for students to stain for phalloidin
paraglut fixed 3t3's for students to stain for phalloidin
live LL's to fix in paraglut (2014: treat first with mitotracker, then fix in formaldehyde no detergent)
Solutions
PBS to rinse coverslips
2% PBS-BSA to make up antibodies
Materials
forceps
50 mL beakers (switch to holders and 100 mL beakers?)
petri dishes for humid chambers
filter paper for humid chambers
mounting medium
slides
nail polish
kimwipes
parafilm