Bradykinin for 6.2
Bradykinin for 6.2 kdorfman Mon, 11/04/2013 - 17:191mg/mL Bradykinin stock
(= 1.0602 mM) Sigma B3259 - 1 mg septum bottle
MW = 106.02
Add 1 mL H2O to the 1 mg in the bottle (NO CALCIUM! - some will be used for the calcium experiments in Lab 7.2)
2 µM Bradykinin in HBS
Use bradykinin aliquots from Lab 6.1
2 µM Bradykinin, Calcium-free
(=2.12 µg/mL)
Lab 6.2: 0.75 mL per coverslip x 2 coverslips x 8 groups = 12 mL
~2 µL 1 mg/mL stock per 1 mL working solution
31.8 µL 1 mg/mL stock in 15 mL Ca-free HBS
Make 8 aliquots of 1.6 mL
Make more if necessary. (Aliquots should have been enough for 15 mL?)