from stock solutions

10X PBS from stock solutions

from Sigma ready-made 1X:

M (1X)	M (10X)	ingredient
0.01	0.10	Na-K Phosphate
0.138	1.38	NaCl
0.0027	0.027	KCl

calculated from Carrie's dry ingredients recipe:

To make	L 10X PBS
add:	mL 5M NaCl
add:	mL 1M KCl
add:	mL 1M KH2PO4
add:	mL 1M Na2HPO4.7 H2O (MW = 268.07)

pH to 7.3 with NaOH

sterilize

---

OR

To match Sigma recipe:

Make 1M Na2HPO4 (base); 1 M KH2PO4 (acid)

Mix to pH 7.3

(should be ~38.25 mL Na2HPO4, 11.5 mL KH2PO4) from the Sigma Buffer Reference Center

pH	mL Na Phos dibasic	mL Na Phos monobasic
7.2	36.0	14.0
7.4	40.5	9.5

Then

To make	L 10X PBS
add:	mL 5M NaCl
add:	mL 1M KCl
add:	mL 1M K-Na Phosphate