hydroponicshydroponics kdorfman Thu, 05/22/2014 - 21:33
Make 1x MS, pH to 5.6
Split: most as growth medium, a little bit for 0.7% agar (err on the low end), sterilize
Cut lids off microfuge tubes, poke a hole in each, sterilize in a beaker
line lids up, flat side down, on a strip of scotch tape
fill with 0.7% agar.
put lids into floatie or other rack.
put 1 sterilized seed in each hole. (in 0.1% agar)
Wrap with saran wrap
Cover tightly with foil, stratify in refrigerator for 2-3 days
Remove foil, put container in growth chamber at 16h day, 22C
Transfer lid to 50 mL tube with hole drilled in it at ~3 weeks.
Aerate or stir medium