MV

Minimal Vitamin Medium for Bio 284

Autoclave an appropriately sized graduated cylinder to measure the glucose solution.

To make	MV agar plates
pour	mm plate diameter
pour	mL per plate
start with:	mL water
add:	g YNB*, stir till dissolved
add:	g ammonium sulfate, stir till dissolved
add:	g agar, leave stir bar in
autoclave for:	minutes
add asceptically	mL filter sterilized 20% glucose
for a final volume of	mL

*Difco Yeast Nitrogen Base w/o Amino Acids and w/o Ammonium Sulfate

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